Analysis Note

ControlIncluded free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Research CategoryNeuroscience

Research Sub CategoryIon Channels & TransportersSignaling Neuroscience

Western blot: 1:200 using ECL on ND7/23 cell lysate (mouse neuroblastoma x rat DRG neuron hybrid cell line).
Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Anti-Cav3.2 Antibody is an antibody against Cav3.2 for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified peptide from amino acids 581-595 of rat Ca_v3.2

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Specificity

Recognizes Ca_v3.2 (α1H (T-Type)).

The immunogen sequence is 14 of 15 amino acids identical in mouse, bovine and canine and 13 of 15 amino acids identical in human.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.